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40/D11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 40/D11
NCBI Gene ID 2540133
Gene Symbol php5
Protein Name CCAAT-binding factor complex subunit Php5
Synonym SPBC3B8.02
RefSeq Protein NP_596412.1
PomBase(1) SPBC3B8.02
KEGG(2) spo:SPBC3B8.02
Depositor-supplied data(3) 40D11
Note 165T:C / 207T:C / 1219G:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW016083
Clone name SpENT40D11
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW056083
Clone name SpFFH40D11
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW096083
Clone name SpYFH40D11
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH40D11a.seq SpYFH40D11b.seq   SpYFH40D11.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl