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40/C04 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 40/C04
NCBI Gene ID 2540547
Gene Symbol tdh1
Protein Name glyceraldehyde-3-phosphate dehydrogenase Tdh1
Synonym SPBC32F12.11|gpd1
RefSeq Protein NP_596154.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW016052
Clone name SpENT40C04
Clone status confirmation required

FFH Vector(5)

Catalog number SPW056052
Clone name SpFFH40C04
Clone status confirmation required

YFH Vector(6)

Catalog number SPW096052
Clone name SpYFH40C04
Clone status confirmation required
user report - reference Zhao, D., Atg20- and Atg24-family proteins promote organelle autophagy in fission yeast. J. Cell Sci. 129 (22): 4289-4304 (2016). PMID 27737912.
user report - reference Sun, L.L., Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9 (8): e1003715 (2013). PMID 23950735.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


pombe_clone_primer_ver2_160708_xlsx.csv -