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39/H12 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 39/H12
NCBI Gene ID 2540899
Gene Symbol arf1
Protein Name ADP-ribosylation factor, Arf family Arf1
Synonym SPBC4F6.18c
RefSeq Protein NP_596118.1
PomBase(1) SPBC4F6.18c
KEGG(2) spo:SPBC4F6.18c
Depositor-supplied data(3) 39H12
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW015780
Clone name SpENT39H12
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW055780
Clone name SpFFH39H12
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW095780
Clone name SpYFH39H12
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH39H12a.seq SpYFH39H12b.seq   SpYFH39H12.pdf
user report - reference Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl