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36/C12 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 36/C12
NCBI Gene ID 2540353
Gene Symbol SPBC2F12.12c
Protein Name hypothetical protein
Synonym
RefSeq Protein NP_595704.1
PomBase(1) SPBC2F12.12c
KEGG(2) spo:SPBC2F12.12c
Depositor-supplied data(3) 36C12
Note 541A:G / 1128T:A(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW014460
Clone name SpENT36C12
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW054460
Clone name SpFFH36C12
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW094460
Clone name SpYFH36C12
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH36C12a.seq SpYFH36C12b.seq   SpYFH36C12.pdf
user report - reference Lorenzi, L.E., Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels. EMBO J. 34 (1): 115-129 (2015). PMID 25398909.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl