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35/H09 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 35/H09
NCBI Gene ID 2543028
Gene Symbol isp5
Protein Name amino acid permease Isp5
Synonym SPAC1039.09
RefSeq Protein NP_595000.1
PomBase(1) SPAC1039.09
KEGG(2) spo:SPAC1039.09
Depositor-supplied data(3) 35H09
Note 26T:C / 1436T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW014177
Clone name SpENT35H09
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW054177
Clone name SpFFH35H09
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW094177
Clone name SpYFH35H09
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH35H09a.seq SpYFH35H09b.seq   SpYFH35H09.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl