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34/C12 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 34/C12
NCBI Gene ID 2543344
Gene Symbol cut6
Protein Name acetyl-CoA/biotin carboxylase
Synonym SPAC56E4.04c
RefSeq Protein NP_593271.1
PomBase(1) SPAC56E4.04c
KEGG(2) spo:SPAC56E4.04c
Depositor-supplied data(3) 34C12
Note 3092A:G / 3204T:C / 4333T:C / 4525T:C / 5204A:G / 5513A:G / 5547A:G / 5662C:A / 6505T:C / 6630T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW013660 SpENT34C12 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW053660 SpFFH34C12 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpFFH34C12a.seq SpFFH34C12b.seq   SpFFH34C12.pdf
user_report - reference Prevorovsky, M., CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast. Cell Cycle 15 (22): 3082-3093 (2016). PMID 27687771.

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW093660 SpYFH34C12 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


pombe_clone_primer_ver2_2023Apr26.csv -