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34/B04 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 34/B04
NCBI Gene ID 2540148
Gene Symbol dna2
Protein Name DNA replication endonuclease-helicase Dna2
Synonym SPBC16D10.04c
RefSeq Protein NP_596499.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note 1208A:G / 1690A:G / 2850T:C / 3985A:G(4)

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW013628
Clone name SpENT34B04
Clone status confirmation required

FFH Vector(5)

Catalog number SPW053628
Clone name SpFFH34B04
Clone status confirmation required

YFH Vector(6)

Catalog number SPW093628
Clone name SpYFH34B04
Clone status confirmation required
user report - reference Zhang, J.M., Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2. PLoS Biol. 12 (9): e1001946 (2014). PMID 25203555.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.01.30

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl