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31/A11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 31/A11
NCBI Gene ID 3361214
Gene Symbol atg2
Protein Name autophagy associated protein Mug36
Synonym SPBC31E1.01c|SPBC660.18c
RefSeq Protein
PomBase(1) SPBC31E1.01c
KEGG(2) spo:SPBC31E1.01c
Depositor-supplied data(3) 31A11
Note  

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
  SpENT31A11 not available

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW052411 SpFFH31A11 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpFFH31A11a.seq SpFFH31A11b.seq   SpFFH31A11.pdf
user report - reference Osawa, T., Atg2 mediates direct lipid transfer between membranes for autophagosome formation. Nat. Struct. Mol. Biol. 26 (4): 281-288 (2019). PMID 30911189.

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW092411 SpYFH31A11 confirmation required
user report - reference Sun, L.L., Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9 (8): e1003715 (2013). PMID 23950735.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2019.12.24

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_191224.pl