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30/G11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 30/G11
NCBI Gene ID 2540746
Gene Symbol SPBC211.03c
Protein Name guanyl-nucleotide exchange factor (predicted)
Synonym
RefSeq Protein NP_596613.1
PomBase(1) SPBC211.03c
KEGG(2) spo:SPBC211.03c
Depositor-supplied data(3) 30G11
Note 546A:G / 2547A:G / 2874T:C / 3932T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW012155
Clone name SpENT30G11
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT30G11a.seq SpENT30G11b.seq   SpENT30G11.pdf

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW052155
Clone name SpFFH30G11
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW092155
Clone name SpYFH30G11
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH30G11a.seq SpYFH30G11b.seq   SpYFH30G11.pdf
user report - reference Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl