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30/F09 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 30/F09
NCBI Gene ID 2538930
Gene Symbol dcr1
Protein Name dicer
Synonym SPCC188.13c|SPCC584.10c
RefSeq Protein NP_588215.2
PomBase(1) SPCC188.13c
KEGG(2) spo:SPCC188.13c
Depositor-supplied data(3) 30F09
Note 264A:G / 579A:G / 3700T:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW012129
Clone name SpENT30F09
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW052129
Clone name SpFFH30F09
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW092129
Clone name SpYFH30F09
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH30F09a.seq SpYFH30F09b.seq   SpYFH30F09.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl