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30/C02 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 30/C02
NCBI Gene ID 2539712
Gene Symbol med15
Protein Name mediator complex subunit Med15 (predicted)
Synonym SPBC146.01|SPBP35G2.15
RefSeq Protein
PomBase(1) SPBC146.01
KEGG(2) spo:SPBC146.01
Depositor-supplied data(3) 30C02
Note 1970A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW012050 SpENT30C02 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW052050 SpFFH30C02 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW092050 SpYFH30C02 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH30C02a.seq SpYFH30C02b.seq   SpYFH30C02.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.07

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl