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30/A11 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 30/A11
NCBI Gene ID 2539248
Gene Symbol hrr1
Protein Name Helicase Required for RNAi-mediated heterochromatin assembly Hrr1
Synonym SPCC1739.03
RefSeq Protein NP_588411.1
PomBase(1) SPCC1739.03
KEGG(2) spo:SPCC1739.03
Depositor-supplied data(3) 30A11
Note 449C:T / 611A:G / 2057T:C / 3126A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW012011
Clone name SpENT30A11
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW052011
Clone name SpFFH30A11
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW092011
Clone name SpYFH30A11
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH30A11a.seq SpYFH30A11b.seq   SpYFH30A11.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl