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28/G08 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 28/G08
NCBI Gene ID 2540050
Gene Symbol sec27
Protein Name coatomer beta' subunit (predicted)
Synonym SPBC16C6.13c
RefSeq Protein NP_596811.1
PomBase(1) SPBC16C6.13c
KEGG(2) spo:SPBC16C6.13c
Depositor-supplied data(3) 28G08
Note 1208T:C / 1828T:C / 2324T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW011352
Clone name SpENT28G08
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpENT28G08a.seq SpENT28G08b.seq   SpENT28G08.pdf

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW051352
Clone name SpFFH28G08
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW091352
Clone name SpYFH28G08
Clone status confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl