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27/F12 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 27/F12
NCBI Gene ID 2543281
Gene Symbol rad4
Protein Name BRCT domain protein Rad4
Synonym SPAC23C4.18c|cut5,dre3
RefSeq Protein NP_593190.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note 2062A:deletion(4)

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW010932
Clone name SpENT27F12
Clone status confirmation required

FFH Vector(5)

Catalog number SPW050932
Clone name SpFFH27F12
Clone status confirmation required

YFH Vector(6)

Catalog number SPW090932
Clone name SpYFH27F12
Clone status confirmation required
user report - reference Qu, M., Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint. PLoS Genet. 8 (7): e1002817 (2012). PMID 22792081.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


pombe_clone_primer_ver2_160708_xlsx.csv -