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26/D11 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 26/D11
NCBI Gene ID 2538725
Gene Symbol SPCC825.02
Protein Name glucosidase II Gtb1 (predicted)
RefSeq Protein NP_588052.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note 1397A:G(4)

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW010483
Clone name SpENT26D11
Clone status confirmation required
user report - reference Olson, L.J., Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition. Biochemistry 54 (26): 4097-4111 (2015). PMID 26062005.
user report - reference Olson, L.J., Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum. J. Biol Chem. ;288 (23): 16460-16475 (2013). PMID 23609449.
user report - reference Stigliano, I.D., Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo. Mol. Biol. Cell 22 (11): 1810-1823 (2011). PMID 21471007.
user report - reference Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

FFH Vector(5)

Catalog number SPW050483
Clone name SpFFH26D11
Clone status confirmation required

YFH Vector(6)

Catalog number SPW090483
Clone name SpYFH26D11
Clone status confirmation required

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


pombe_clone_primer_ver2_160708_xlsx.csv -