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25/C12 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 25/C12
NCBI Gene ID 2541478
Gene Symbol SPAC2F7.17
Protein Name mitochondrial peptide chain release factor (predicted)
Synonym
RefSeq Protein NP_592987.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note 506T:addition / 535A:G / 1160G:A / 1504C:T(4)

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW010060
Clone name SpENT25C12
Clone status confirmation required

FFH Vector(5)

Catalog number SPW050060
Clone name SpFFH25C12
Clone status confirmation required

YFH Vector(6)

Catalog number SPW090060
Clone name SpYFH25C12
Clone status confirmation required
user report - reference Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.01.30

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl