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21/B04 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 21/B04
NCBI Gene ID 2542427
Gene Symbol SPAC6F6.12
Protein Name autophagy associated protein Atg24 (predicted)
Synonym
RefSeq Protein NP_593905.1
PomBase(1) SPAC6F6.12
KEGG(2) spo:SPAC6F6.12
Depositor-supplied data(3) 21B04
Note 314T:C / 668A:G / 1131T:C(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW008428 SpENT21B04 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW048428 SpFFH21B04 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW088428 SpYFH21B04 confirmation required
user_report - reference Zhao, D., Atg20- and Atg24-family proteins promote organelle autophagy in fission yeast. J. Cell Sci. 129 (22): 4289-4304 (2016). PMID 27737912.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.01

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl