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20/E03 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 20/E03
NCBI Gene ID 2540215
Gene Symbol rfc5
Protein Name DNA replication factor C complex subunit Rfc5 (predicted)
Synonym SPBC83.14c
RefSeq Protein NP_595646.1
PomBase(1) SPBC83.14c
KEGG(2) spo:SPBC83.14c
Depositor-supplied data(3) 20E03
Note 1215T:C / 1268A:T(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW008099 SpENT20E03 confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW048099 SpFFH20E03 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW088099 SpYFH20E03 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2023.11.01

pombe_clone_primer_ver2_2023Apr26.csv - GNP_filter3_pombe_html_191224.pl