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18/E01 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 18/E01
NCBI Gene ID 2543628
Gene Symbol spo20
Protein Name sec14 cytosolic factor family Sec14
Synonym SPAC3H8.10|sec14
RefSeq Protein NP_593003.1
PomBase(1) SPAC3H8.10
KEGG(2) spo:SPAC3H8.10
Depositor-supplied data(3) 18E01

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW007297 SpENT18E01 confirmation required
user_report - reference Dodgson, J., Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation. Eukaryot. Cell, 9, 1788-1797 (2010). PMID 20870879.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW047297 SpFFH18E01 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW087297 SpYFH18E01 confirmation required

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


pombe_clone_primer_ver2_2023Apr26.csv -