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13/E01 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 13/E01
NCBI Gene ID 2539937
Gene Symbol arf6
Protein Name ADP-ribosylation factor, Arf family Arf6
Synonym SPBC1539.08
RefSeq Protein NP_596822.1
PomBase(1) SPBC1539.08
KEGG(2) spo:SPBC1539.08
Depositor-supplied data(3) 13E01
Note 658G:A(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW005297
Clone name SpENT13E01
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW045297
Clone name SpFFH13E01
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW085297
Clone name SpYFH13E01
Clone status available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH13E01a.seq SpYFH13E01b.seq   SpYFH13E01.pdf
user report - reference Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617.

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl