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07/D07 - S. pombe Postgenome Database Clone

Available from Ordering information ordering information
Clone ID at the S. pombe Postgenome Database 07/D07
NCBI Gene ID 2539083
Gene Symbol rpb6
Protein Name DNA-directed RNA polymerase I, II and III subunit Rpb6
Synonym SPCC1020.04c|rpo15
RefSeq Protein NP_587956.1
PomBase(1) link 01
KEGG(2) link 01
Depositor-supplied data(3) link 01
Note 9C:T / 399T:deletion(4)

Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Entry vector

Catalog number SPW002879
Clone name SpENT07D07
Clone status confirmation required

FFH Vector(5)

Catalog number SPW042879
Clone name SpFFH07D07
Clone status confirmation required

YFH Vector(6)

Catalog number SPW082879
Clone name SpYFH07D07
Clone status confirmation required
user report - reference Weston, L., Genome-wide screen for cell growth regulators in fission yeast. J Cell Sci. 2017 May 5. pii: jcs.200865. doi: 10.1242/jcs.200865. [Epub ahead of print] PMID 28476936.

Please note that they are GENOMIC clones.
Please visit plasmidData Sheet for the information of vectors and sequence primers.

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

(5) FLAG2-His6-tag clone.

(6) YFP-FLAG-His6-tag clone.

(7) Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).


2017.06.20

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_160723.pl