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02/G12 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 02/G12
NCBI Gene ID 2539523
Gene Symbol htb1
Protein Name histone H2B alpha Htb1
Synonym SPCC622.09
RefSeq Protein NP_588181.1
PomBase(1) SPCC622.09
KEGG(2) spo:SPCC622.09
Depositor-supplied data(3) 02G12
Note 32A:G / 110A:G(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number Clone name Clone status
SPW000956 SpENT02G12 confirmation required
user_report - reference Dodgson, J., Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation. Eukaryot. Cell, 9, 1788-1797 (2010). PMID 20870879.

FFH Vector (FLAG2-His6-tag clone)

Catalog number Clone name Clone status
SPW040956 SpFFH02G12 confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number Clone name Clone status
SPW080956 SpYFH02G12 available
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH02G12a.seq SpYFH02G12b.seq   SpYFH02G12.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


pombe_clone_primer_ver2_2023Apr26.csv -