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01/F02 - S. pombe Postgenome Database Clone

 Plasmid request
Clone ID at the S. pombe Postgenome Database 01/F02
NCBI Gene ID 2542463
Gene Symbol hhf1
Protein Name histone H4 h4.1
Synonym SPAC1834.03c|h4.1
RefSeq Protein NP_594682.1
PomBase(1) SPAC1834.03c
KEGG(2) spo:SPAC1834.03c
Depositor-supplied data(3) 01F02
Note 9C:T(4)

(1) The PomBase, funded by the Wellcome Trust and is run by a consortium comprising the University of Cambridge, the European Bioinformatics Institute and University College London.

(2) The Kyoto Encyclopedia of Genes and Genomes of the Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo.

(3) Primer sequences for constructing an entry clone and known sequence variation are available at this site, the S. pombe Postgenome Database of the Chemical Geneitcs Laboratory, RIKEN.

(4) Remarks by the Depositor.

Please note that the information was bioinformatically annotated and that this information may not reflect the most recent annotation of the reference sequence. You should carefully check the insert sequence provided by us to make sure that it matches the sequence you expect.
Gene Engineering Division has digested by restriction enzymes and sequenced a portion of the resource for verification. The result is shown below if available.

Please note that they are GENOMIC clones.

Entry vector

Catalog number SPW000522
Clone name SpENT01F02
Clone status confirmation required

FFH Vector (FLAG2-His6-tag clone)

Catalog number SPW040522
Clone name SpFFH01F02
Clone status confirmation required

YFH Vector (YFP-FLAG-His6-tag clone)

Catalog number SPW080522
Clone name SpYFH01F02
Clone status This clone actually corresponds to Hff3 instead of Hff1.
Seq File
(by primer A)
Seq File
(by primer B)
Seq File
(by primer C)
PDF File
SpYFH01F02a.seq SpYFH01F02b.seq   SpYFH01F02.pdf

Reference of clones: Matsuyama, A., Nature Biotech., 24, 841-847 (2006).

Please visit Data Sheet for the information of vectors and sequence primers.


2018.10.13

pombe_clone_primer_ver2_160708_xlsx.csv - GNP_filter3_pombe_html_171016.pl