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pCS-hU6-Neo(V2) (#RDB21099)

Guide RNA expression vector under the control of human U6 (hU6) promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Shimizu, T., Proc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120 (2023). PMID 38085778. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Guide RNA expression vector. The CAG promoter of CS-CA-MCS (RDB05963) was replaced with the human U6 (hU6) promoter, and the gRNA scaffold sequence was cloned downstream of the hU6 promoter to generate pCS-hU6. The kanamycin/neomycin-resistance gene was PCR amplified from pCAG-HIVgp and cloned downstream of the sgRNA scaffold sequence of pCS-hU6 to generate pCS-hU6-Neo(V2).
Vector backbone CS-CA-MCS (RDB05963) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB, 37℃
Depositor|Developer Okae, Hiroaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Shimizu, T. et al. Proc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120, 2023). For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Kumamoto University (kico-mta@jimu. kumamoto-u.ac.jp)
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimizu, T. et al. Proc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120, 2023)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、熊本大学 (kico-mta@jimu. kumamoto-u.ac.jp) の許可を得ること。
付加的使用条件：
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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RDB21099 pCS-hU6-Neo(V2) DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCS-hU6-Neo(V2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB21099).

Reference section:

Shimizu, T., Oike, A., Kobayashi, E.H., Sekiya, A., Kobayashi, N., Shibata, S., Hamada, H., Saito, M., Yaegashi, N., Suyama, M., Arima, T., Okae, H., CRISPR screening in human trophoblast stem cells reveals both shared and distinct aspects of human and mouse placental development. Proc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120 (2023). PMID 38085778. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shimizu, T., CRISPR screening in human trophoblast stem cells reveals both shared and distinct aspects of human and mouse placental development. Proc. Natl. Acad. Sci. U.S.A. 120 (51): e2311372120 (2023). PMID 38085778. [PubMed] [Article] [RRC of NBRP]