Resource data sheet
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pCAG:NCad-B1-P2A-miRFP670-NLS (#RDB20994)

The expression vector of ddFP-B1, which is part of the N-cadherin interaction indicator.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kanadome, T., Commun. Biol. 5 (1): 1065 (2022). PMID 36207396. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. The expression vector contains a mouse N-cadherin with an inserted ddFP-B1, which is part of the N-cadherin interaction indicator. The near-infrared fluorescent protein miRFP670 is expressed in a polycistronic manner via the P2A peptide and localizes to the nucleus. Use in combination with pCAG:NCad-GA-P2A-mCherry-NLS (RDB20993).
Vector backbone pCAGGS1 (plasmid)
Growth conditions Ampicillin, 100 μg/mL, 37°C
Gene/insert name mouse Cdh2 cDNA; Discosoma sp. ddFP-B1; porcine teschovirus-1 self-cleaving sequence P2A; Rhodopseudomonas palustris fluorescent protein (miRFP670); SV40 NLS.
Depositor|Developer Matsuda, Tomoki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Kanadome T. et al. Commun. Biol., 5(1):1065. doi: 10.1038/s42003-022-04023-2. 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Kitasato University 　　(ksuisin@kitasato-u.ac.jp).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kanadome T. et al. Commun. Biol., 5(1):1065. doi: 10.1038/s42003-022-04023-2. 2022)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、北里大学 (ksuisin@kitasato-u.ac.jp)の許可を得ること。
付加的使用条件：
CAG プロモータを含むリソースについて: 利用者は、研究成果の公表にあたって CAG プロモータの文献(Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB20994 pCAG:NCad-B1-P2A-miRFP670-NLS DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG:NCad-B1-P2A-miRFP670-NLS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20994).

Reference section:

Kanadome, T., Hayashi, K., Seto, Y., Eiraku, M., Nakajima, K., Nagai, T., Matsuda, T., Development of intensiometric indicators for visualizing N-cadherin interaction across cells. Commun. Biol. 5 (1): 1065 (2022). PMID 36207396. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kanadome, T., Development of intensiometric indicators for visualizing N-cadherin interaction across cells. Commun. Biol. 5 (1): 1065 (2022). PMID 36207396. [PubMed] [Article] [RRC of NBRP]