Resource data sheet
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pCSII-CMV-luc-IRES2-Bsd (#RDB20794)

Lentivirus vector capable of expressing firefly luciferase.

Clone info. Lentivirus vector capable of expressing firefly luciferase. See Materials and Methods of Suzuki N, et al., Sci. Rep., 8(1):15036, 2018. doi: 10.1038/s41598-018-33042-5.
Vector backbone CSII-CMV-MCS-IRES2-Bsd (RDB04385) (plasmid)
Selectable markers Ampicillin (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Photinus pyralis luciferase gene from a pGL2 series vector (Promega Corp.)
Depositor|Developer Yamaoka, Shoji |
 
Sequence (full) RDB20794hts01.seq checkAssembled from experimentally sequenced data.
Publication Suzuki, N., Sci. Rep. 8 (1): 15036 (2018). PMID 30310119. [link to RRC of NBRP]
Test sheet RDB20794C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Suzuki et al. Scientific Reports 8:15036, 2018) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for not-for-profit research in the not-for-profit organization.
Additional terms and conditions:
Regarding luciferase: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki et al. Scientific Reports 8:15036, 2018)。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
付加的使用条件：
ルシフェラーゼについて：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20794 pCSII-CMV-luc-IRES2-Bsd DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCSII-CMV-luc-IRES2-Bsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20794).

Reference section:

Suzuki, N., Yoshida, T., Takeuchi, H., Sakuma, R., Sukegawa, S., Yamaoka, S., Robust Enhancement of Lentivirus Production by Promoter Activation. Sci. Rep. 8 (1): 15036 (2018). PMID 30310119. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suzuki, N., Robust Enhancement of Lentivirus Production by Promoter Activation. Sci. Rep. 8 (1): 15036 (2018). PMID 30310119. [link to RRC of NBRP]