Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pGL3-mUcp1Enhancer_MiniPromoter_Luciferase (#RDB20463)

Mouse Ucp1 -2.5kb enhancer region plus a minimal promoter sequence in the pGL3-basic luciferase vector.

Clone info. Mouse Ucp1 -2.5kb enhancer region is cloned with the minimal promoter sequence (pGL4.24 vector sequence) in the pGL3-basic luciferase vector.
Vector backbone pGL3-basic (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Ampicillin, 37oC
Depositor|Developer Kim, Jae Bum |
 
Sequence (full) RDB20463hts01.seq checkAssembled from experimentally sequenced data.
Publication Jeon, Y.G., Nat. Commun. 15 (1): 940 (2024). [link to RRC of NBRP]
Test sheet RDB20463C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution BIOLOGICAL RESOURCE to a for-profit orga1lization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Seoul National University R&DB Foundation. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yong Geun Jeon et al. Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates. Nat. Commun. 15(1): 940, 2024.)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 BIOLOGICAL RESOURCE to a for-profit orga1lization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Seoul National University R&DB Foundation. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yong Geun Jeon et al. Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates. Nat. Commun. 15(1): 940, 2024.)
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20463 pGL3-mUcp1Enhancer_MiniPromoter_Luciferase DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGL3-mUcp1Enhancer_MiniPromoter_Luciferase was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20463).

Reference section:

Jeon, Y.G., Nahmgoong, H., Oh, J., Lee, D., Kim, D.W., Kim, J.E., Kim, Y.Y., Ji, Y., Han, J.S., Kim, S.M., Sohn, J.H., Lee, W.T., Kim, S.W., Park, J., Huh, J.Y., Jo, K., Cho, J.Y., Park, J., Kim, J.B., Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates. Nat. Commun. 15 (1): 940 (2024). PMID 38296968. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Jeon, Y.G., Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates. Nat. Commun. 15 (1): 940 (2024). PMID 38296968. [link to RRC of NBRP]