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pRSETA-qmTQ2-glucose (#RDB20452)

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose.

Clone info. Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose. See Zhong, C. et al., Cell Rep. Methods 4 (11): 100902 (2024). DOI: 10.1016/j.crmeth.2024.100902
Comment Fig. 3B: Schematic domain structure of the qmTQ2-citrate biosensor.
Vector backbone pRSETA (plasmid)
Selectable markers Ampicillin (E coli)
Growth conditions LB+Ampicillin, 37 °C
Growth remarks Recommended E. coli host for plamid amplication, DH5a
Gene/insert name Escherichia coli mglB genomic DNA; Aequorea victoria fluorescent protein (mTurquoise2).
Depositor|Developer Okada, Yasushi | Zhong, Chongxia |
 
Sequence (full) RDB20452hts01.seq checkAssembled from experimentally sequenced data.
Publication Unpublished bioresource
Test sheet RDB20452C0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Zhong, C. et al., Cell Rep. Methods 4 (11): 100902, 2024.) For use by for-profit organizations or for-profit research use by non-profit academic institutions, permission must be obtained from RIKEN BDR.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Zhong, C. et al., Cell Rep. Methods 4 (11): 100902, 2024.)。 営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研BDRの許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20452 pRSETA-qmTQ2-glucose DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pRSETA-qmTQ2-glucose was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20452).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Zhong, C., Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Cell Rep. Methods 4 (11): 100902 (2024). PMID 39561716. [link to RRC of NBRP]