Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate.
Clone info. | Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate. See Zhong, C. et al., Cell Rep. Methods 4 (11): 100902 (2024). DOI: 10.1016/j.crmeth.2024.100902 |
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Comment | Fig. 3A: Schematic domain structure of the qmTQ2-citrate biosensor. |
Vector backbone | pRSETA (plasmid) |
Selectable markers | Ampicillin (E coli) |
Growth conditions | LB+Ampicillin, 37 °C |
Growth remarks | Recommended E. coli host for plamid amplication, DH5a |
Gene/insert name | Klebsiella pneumoniae CitA genomic DNA; Aequorea victoria fluorescent protein (mTurquoise2). |
Depositor|Developer | Okada, Yasushi | Zhong, Chongxia | |
Sequence (full) | RDB20451hts01.seq ![]() |
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Publication | Unpublished bioresource |
Test sheet | RDB20451C0p1-1.pdf ![]() |
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Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Zhong, C. et al., Cell Rep. Methods 4 (11): 100902, 2024.) For use by for-profit organizations or for-profit research use by non-profit academic institutions, permission must be obtained from RIKEN BDR. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Zhong, C. et al., Cell Rep. Methods 4 (11): 100902, 2024.)。 営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研BDRの許可を得ること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB20451 | pRSETA-qmTQ2-citrate | DNA solution |
Materials & Methods section:
The pRSETA-qmTQ2-citrate was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20451). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
user_report | Zhong, C., Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Cell Rep. Methods 4 (11): 100902 (2024). PMID 39561716. [link to RRC of NBRP] |
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