Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCAGGS-BLRP-hRXRα-IRES-BirA (#RDB20443)

Expression vector of human RXR alpha tagged with BLRP for a modified ChIP analysis using streptavidin beads.

Clone info. Co-expression vector of N-terminally BLRP tagged human RXR alpha and BirA. Biotin is added to the BLRP sequence of human RXR alpha by BirA and can be used for modified ChIP analysis using streptavidin beads. Komai, M. et al., J. Lipid Res., 65 (3):100510, 2024 (DOI 10.1016/j.jlr.2024.100510).
Vector backbone pCAGGS-BLRP-IRES-BirA (RDB15774) (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human RXRA cDNA.
Depositor|Developer Takasugi, Nobumasa |
 
Sequence (full) RDB20443hts01.seq checkAssembled from experimentally sequenced data.
Publication Komai, M., J. Lipid Res. 65 (3): 100510 (2024). [link to RRC of NBRP]
Test sheet RDB20443C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Masato K. et al., J. Lipid Res. 65(3):100510, 2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the okayama University.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masato K. et al., J. Lipid Res. 65(3):100510, 2024)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、岡山大学の許可を得ること。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20443 pCAGGS-BLRP-hRXRα-IRES-BirA DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAGGS-BLRP-hRXRα-IRES-BirA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20443).

Reference section:

Komai, M., Noda, Y., Ikeda, A., Kaneshiro, N., Kamikubo, Y., Sakurai, T., Uehara, T., Takasugi, N., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Komai, M., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]