Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pET-mDicalcin (#RDB20438)

Expression vector of mouse dicalcin in pET17b vector.

Alternative name mouse dicalcin in pET vector
Clone info. Expression vector of mouse dicalcin in pET17b vector.
Comment It is recommended that the recombinant DNA obtained after amplification is used to express the protein using BL21 pLysS. See Figure 2B of Hanaue, M. et al. Mol. Reprod. Dev., 78(2), 91–103, 2011 (DOI: 10.1002/mrd.21273), or Figure 1 of Miwa, N. et al. Biochem. Biophys. Res. Commun., 251(3), 860–867, 1998 (DOI: 10.1006/bbrc.1998.9571).
Vector backbone pET17b (plasmid)
Gene/insert name mouse Dicalcin (S100a11) cDNA.
Depositor|Developer Miwa, Naofumi |
 
Sequence (full) RDB20438hts01.seq checkAssembled from experimentally sequenced data.
Publication Miwa, N., Commun. Biol. 6 (1): 1015 (2023). [link to RRC of NBRP]
Test sheet RDB20438C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miwa N. et al. Communications Biolgy 6,1015, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Saitama Medical University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miwa N. et al. Communications Biolgy 6,1015, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、埼玉医科大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20438 pET-mDicalcin DNA solution


How to cite this biological resource

Materials & Methods section:

The pET-mDicalcin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20438).

Reference section:

Miwa, N., Hanaue, M., Aoba, K., Saito, R., Takamatsu, K., Dicalcin suppresses invasion and metastasis of mammalian ovarian cancer cells by regulating the ganglioside-Erk1/2 axis. Commun. Biol. 6 (1): 1015 (2023). PMID 37803211. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Miwa, N., Dicalcin suppresses invasion and metastasis of mammalian ovarian cancer cells by regulating the ganglioside-Erk1/2 axis. Commun. Biol. 6 (1): 1015 (2023). PMID 37803211. [link to RRC of NBRP]
reference Hanaue, M., Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract. Mol. Reprod. Dev. 78 (2): 91-103 (2011). PMID 21254279.