Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCMV-SVF-P2A-VSV-G-pRSV-REV (#RDB20434)

Sendai Virus Fusion (SVF) protein-containing lentivirus packaging plasmid.

Clone info. Sendai Virus Fusion (SVF) protein-containing lentivirus packaging plasmid. See Figure S1D of Noguchi, Y. et al. Cell Genom., 4 (3): 100510, 2024.
Vector backbone pCMV-VSV-G-RSV-REV (RDB04393) (plasmid)
Gene/insert name VSV G protein, HIV-1 Rev, Sendai Virus F (fusion)
Reference of insert sequence GenBank: U86411.1 (DDBJ accession number)
Depositor|Developer Suzuki, Jun |
 
Sequence (full) RDB20434hts01.seq checkAssembled from experimentally sequenced data.
Publication Noguchi, Y., Cell Genom. 4 (3): 100510 (2024). [link to RRC of NBRP]
Test sheet RDB20434C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The exportation of this material to foreign countries is controlled and restricted by the law of Japan. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Noguchi, Y. et al., Cell Genom. 4(3):10051, 2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Kyoto University.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Currently unavailable outside Japan. Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Noguchi, Y. et al., Cell Genom. 4(3):10051, 2024)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、京都大学の許可を得ること。本件リソースの輸出は法令等により規制されている。
付加的使用条件：
本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20434 pCMV-SVF-P2A-VSV-G-pRSV-REV DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCMV-SVF-P2A-VSV-G-pRSV-REV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20434).

Reference section:

Noguchi, Y., Onodera, Y., Miyamoto, T., Maruoka, M., Kosako, H., Suzuki, J., In vivo CRISPR screening directly targeting testicular cells. Cell Genom. 4 (3): 100510 (2024). PMID 38447574. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Noguchi, Y., In vivo CRISPR screening directly targeting testicular cells. Cell Genom. 4 (3): 100510 (2024). PMID 38447574. [link to RRC of NBRP]
reference Noguchi, Y., Protocol for in vivo CRISPR screening targeting murine testicular cells. STAR Protoc. 5 (3): 103306 (2024). PMID 39269899. [link to RRC of NBRP]