RDB20429 - CS2-CMV-nGRAPHIC-P2A-mCherry

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CS2-CMV-nGRAPHIC-P2A-mCherry (#RDB20429)

Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide.

Clone info.	Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide.
Vector backbone	CSII-CMV-MCS (RDB04377) (plasmid)
Selectable markers	Ampicillin
Growth remarks	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Kuchimaru, Takahiro \|

Sequence (full)	RDB20429hts01.seq Assembled from experimentally sequenced data.
Publication	Unpublished bioresource
Test sheet	RDB20429C0p1-1.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Misa M. et al. Nat Commun. 14(1):8031, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Jichi Medical University.
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Misa M. et al. Nat Commun. 14(1):8031, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Jichi Medical University. Additional terms and conditions: Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Misa M. et al. Nat Commun. 14(1):8031, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、自治医科大学の許可を得ること。付加的使用条件：レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB20429	CS2-CMV-nGRAPHIC-P2A-mCherry	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein