Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide.
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| Clone info. | Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide. |
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| Vector backbone | CSII-CMV-MCS (RDB04377) (plasmid) |
| Selectable markers | Ampicillin |
| Growth remarks | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Depositor|Developer | Kuchimaru, Takahiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment In order to receive this biological resource, please use the Exclusive MTA [Link]. Please visit Ordering instruction.[link] |
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| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Minegishi, M. et al. Nat Commun. 14(1):8031, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Jichi Medical University. Additional terms and conditions: Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Further transfer, provision of services or resale of the Zeocin resistance gene, and the Deposited Materials is prohibited without InvivoGen's prior written consent. In addition, InvivoGen's Limited Use Label License applies to your use of this product. |
| Remarks | Remember that you will be working with samples containing infectious virus. Zeocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents. See InvivoGen Limited Use Label License. |
[open/close]| 必要書類 | 提供依頼書 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Minegishi, M. et al. Nat Commun. 14(1):8031, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、自治医科大学の許可を得ること。 付加的使用条件: レンチウイルスベクターについて:本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。 InvivoGen社の事前の書面による同意がない限り、ゼオシン耐性遺伝子および寄託マテリアルの第三者への移転、サービスの提供、または再販売は禁止されています。また、ご利用に際してInvivoGen社のLimited Use Label Licenseが適用されます。 |
| 備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 ZeocinはInvivoGen社の製品です。Zeocin®は、米国特許商標庁および米国外の同等機関に登録されたInvivoGen社の商標です。InvivoGen Limited Use Label Licenseをご確認ください。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB20429 | CS2-CMV-nGRAPHIC-P2A-mCherry | DNA solution |
Materials & Methods section:
| The CS2-CMV-nGRAPHIC-P2A-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20429). |
Reference section:
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