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CS2-CMV-scGRAPHIC-T2A-H2B-Azurite (#RDB20427)

Expression vector of c-terminal sfGFP with secretion peptide, co-expression of human histone 2B (H2B)-Azurite via T2A peptide.

Clone info. Expression vector of c-terminal sfGFP with secretion peptide, co-expression of human histone 2B (H2B)-Azurite via T2A peptide.
Vector backbone CSII-CMV-MCS (RDB04377) (plasmid)
Selectable markers Ampicillin
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Kuchimaru, Takahiro |
 
Sequence (full) RDB20427hts01.seq checkAssembled from experimentally sequenced data.
Publication Minegishi, M., Nat. Commun. 14 (1): 8031 (2023). PMID 38052804. [link to RRC of NBRP]
Test sheet RDB20427C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Misa M. et al. Nat Commun. 14(1):8031, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Jichi Medical University.
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Misa M. et al. Nat Commun. 14(1):8031, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、自治医科大学の許可を得ること。
付加的使用条件：
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20427 CS2-CMV-scGRAPHIC-T2A-H2B-Azurite DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS2-CMV-scGRAPHIC-T2A-H2B-Azurite was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20427).

Reference section:

Minegishi, M., Kuchimaru, T., Nishikawa, K., Isagawa, T., Iwano, S., Iida, K., Hara, H., Miura, S., Sato, M., Watanabe, S., Shiomi, A., Mabuchi, Y., Hamana, H., Kishi, H., Sato, T., Sawaki, D., Sato, S., Hanazono, Y., Suzuki, A., Kohro, T., Kadonosono, T., Shimogori, T., Miyawaki, A., Takeda, N., Shintaku, H., Kizaka-Kondoh, S., Nishimura, S., Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches. Nat. Commun. 14 (1): 8031 (2023). PMID 38052804. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Minegishi, M., Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches. Nat. Commun. 14 (1): 8031 (2023). PMID 38052804. [link to RRC of NBRP]