Resource data sheet
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pAAV2 SynTetOff-EGFP (#RDB20349)

Strong and neuron-specific expression vector of EGFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sohn, J., PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Strong and neuron-specific expression vector of EGFP.
Vector backbone pAAV-MCS (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria fluorescent protein (EGFP).
Depositor|Developer Hioki, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Sohn, J. et. al., PLoS One, 12 (1) :e0169611, 2017) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Juntendo University.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sohn, J. et. al., PLoS One, 12 (1) :e0169611, 2017)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、順天堂大学の許可を得ること。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20349 pAAV2 SynTetOff-EGFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pAAV2 SynTetOff-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20349).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [PubMed] [Article] [RRC of NBRP]