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pUCtk2X2NN_MBP-2xLG3-TEZ (#RDB20338)

Homology donor vector for MBP gene fused with tdTomato fluorescent protein at C-terminal.

Clone info. Homology donor vector for MBP gene fused with tdTomato fluorescent protein at C-terminal.
Comment Left side HSV TK contains c.203T>C (Leu>Pro), right side HSV TK contains c.454G>C (Ala>Pro).

Left side HDR (homology-directed repair) arm matches to NC_000018.10: 76981499..76980430 except 76981439G/A, 76981334A/T, 76981315C/T, 76981262A/G, 76981216C/T, 76980828C/T. Right side HDR arm matches to NC_000018.10: 76980357..76979362.
Vector backbone pUCtk2X2 (RDB19569) (plasmid)
Gene/insert name human MBP cDNA; Discosoma sp. RFP (tdTomato) cDNA.
Depositor|Developer Hayashi, Yohei |
 
Sequence (full) RDB20338hts01.seq checkAssembled from experimentally sequenced data.
Publication Takagi, D., Stem Cell Res. 79: 103493 (2024). [link to RRC of NBRP]
Test sheet RDB20338C0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR ( ) and an acknowledgment to the DEPOSITOR are requested.
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RDB20338 pUCtk2X2NN_MBP-2xLG3-TEZ DNA solution


How to cite this biological resource

Materials & Methods section:

The pUCtk2X2NN_MBP-2xLG3-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20338).

Reference section:

Takagi, D., Tsukamoto, S., Nakade, K., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Yumoto, N., Kawada, J., Hayata, T., Hayashi, Y., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takagi, D., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP]