Homology donor vector for MBP gene fused with tdTomato fluorescent protein at C-terminal.
Clone info. | Homology donor vector for MBP gene fused with tdTomato fluorescent protein at C-terminal. |
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Comment | Left side HSV TK contains c.203T>C (Leu>Pro), right side HSV TK contains c.454G>C (Ala>Pro). Left side HDR (homology-directed repair) arm matches to NC_000018.10: 76981499..76980430 except 76981439G/A, 76981334A/T, 76981315C/T, 76981262A/G, 76981216C/T, 76980828C/T. Right side HDR arm matches to NC_000018.10: 76980357..76979362. |
Vector backbone | pUCtk2X2 (RDB19569) (plasmid) |
Gene/insert name | human MBP cDNA; Discosoma sp. RFP (tdTomato) cDNA. |
Depositor|Developer | Hayashi, Yohei | |
Sequence (full) | RDB20338hts01.seq ![]() |
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Publication | Takagi, D., Stem Cell Res. 79: 103493 (2024). [link to RRC of NBRP] |
Test sheet | RDB20338C0p1-1.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR ( ) and an acknowledgment to the DEPOSITOR are requested. |
必要書類 | 提供依頼書 ![]() ![]() 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献(決定次第理研BRCで記載)を引用し、謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB20338 | pUCtk2X2NN_MBP-2xLG3-TEZ | DNA solution |
Materials & Methods section:
The pUCtk2X2NN_MBP-2xLG3-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20338). |
Reference section:
Takagi, D., Tsukamoto, S., Nakade, K., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Yumoto, N., Kawada, J., Hayata, T., Hayashi, Y., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Takagi, D., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP] |
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