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pX330-MBP (#RDB20337)

Cas9 and gRNA experession vector targeting MBP gene at C-terminal region.

Clone info. Cas9 and gRNA experession vector targeting MBP gene at C-terminal region.
Vector backbone pX330 (plasmid)
Gene/insert name human MBP synthetic (gRNA)
Depositor|Developer Hayashi, Yohei |
 
Sequence (full) RDB20337hts01.seq checkAssembled from experimentally sequenced data.
Publication Takagi, D., Stem Cell Res. 79: 103493 (2024). [link to RRC of NBRP]
Test sheet RDB20337C0p1-1.pdf 
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR ( ) and an acknowledgment to the DEPOSITOR are requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献（決定次第理研BRCで記載）を引用し、謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20337 pX330-MBP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pX330-MBP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20337).

Reference section:

Takagi, D., Tsukamoto, S., Nakade, K., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Yumoto, N., Kawada, J., Hayata, T., Hayashi, Y., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takagi, D., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP]