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pcDNA5 alpha 2M-delta bait-RFP-GFP-His (#RDB20289)

Expression vector of human alpha-2 macroglobulin without bait domain + RFP (mcherry) + GFP (sfGFP) + 6xHis tag at C-terminus. CMV&tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO).

Clone info. Expression vector of human alpha-2 macroglobulin without bait domain + RFP (mcherry) + GFP (sfGFP) + 6xHis tag at C-terminus. CMV&tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO). See Fig.4 of Tomihari, A. et al., Sci. Rep., 13 (1): 4680, 2023 (DOI 10.1038/s41598-023-31104-x).
Vector backbone pcDNA5 FRT TO (plasmid)
Gene/insert name human A2M cDNA; Aequorea victoria GFP (sfGFP) cDNA; Discosoma sp. RFP (mCherry) cDNA.
Depositor|Developer Itakura, Eisuke |
 
Sequence (full) RDB20289hts01.seq checkAssembled from experimentally sequenced data.
Publication Tomihari, A., Sci. Rep. 13 (1): 4680 (2023). [link to RRC of NBRP]
Test sheet RDB20289C0p1-1.pdf 
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Chiba University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、千葉大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20289 pcDNA5 alpha 2M-delta bait-RFP-GFP-His DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pcDNA5 alpha 2M-delta bait-RFP-GFP-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20289).

Reference section:

Tomihari, A., Kiyota, M., Matsuura, A., Itakura, E., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tomihari, A., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]