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pcDNA5 alpha 2M-ALFA-His (#RDB20288)

Expression vector of human alpha-2 macroglobulin + ALFA tag + 6xHis tag at C-terminus. CMV and tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO).

Clone info. Expression vector of human alpha-2 macroglobulin + ALFA tag + 6xHis tag at C-terminus. CMV and tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO). See Fig.2 of Tomihari, A. et al., Sci. Rep., 13 (1): 4680, 2023 (DOI 10.1038/s41598-023-31104-x).
Vector backbone pcDNA5 FRT TO (plasmid)
Gene/insert name human A2M cDNA.
Depositor|Developer Itakura, Eisuke |
 
Sequence (full) RDB20288hts01.seq checkAssembled from experimentally sequenced data.
Publication Tomihari, A., Sci. Rep. 13 (1): 4680 (2023). [link to RRC of NBRP]
Test sheet RDB20288C0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Chiba University.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、千葉大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20288 pcDNA5 alpha 2M-ALFA-His DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA5 alpha 2M-ALFA-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20288).

Reference section:

Tomihari, A., Kiyota, M., Matsuura, A., Itakura, E., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tomihari, A., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]