Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pcDNA5 alpha 2M-RFP-GFP-His (#RDB20287)

Expression vector of human alpha-2 macroglobulin + RFP (mcherry) + GFP (sfGFP) + 6xHis tag at C-terminus. CMV and tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO).

Clone info. Expression vector of human alpha-2 macroglobulin + RFP (mcherry) + GFP (sfGFP) + 6xHis tag at C-terminus. CMV and tet-on inducible, Flp-in vector (drived from pcDNA5 FRT TO). See Fig.1 of Tomihari, A. et al., Sci. Rep., 13 (1): 4680, 2023 (DOI 10.1038/s41598-023-31104-x).
Vector backbone pcDNA5 FRT TO (plasmid)
Gene/insert name human A2M cDNA; Aequorea victoria GFP (sfGFP) cDNA; Discosoma sp. RFP (mCherry) cDNA.
Depositor|Developer Itakura, Eisuke |
 
Sequence (full) RDB20287hts01.seq checkAssembled from experimentally sequenced data.
Publication Tomihari, A., Sci. Rep. 13 (1): 4680 (2023). [link to RRC of NBRP]
Test sheet RDB20287C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Chiba University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること (Tomihari A. et al. Sci. Rep., 13(1): 4680, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、千葉大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20287 pcDNA5 alpha 2M-RFP-GFP-His DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pcDNA5 alpha 2M-RFP-GFP-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20287).

Reference section:

Tomihari, A., Kiyota, M., Matsuura, A., Itakura, E., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tomihari, A., Alpha 2-macroglobulin acts as a clearance factor in the lysosomal degradation of extracellular misfolded proteins. Sci. Rep. 13 (1): 4680 (2023). PMID 36977730. [link to RRC of NBRP]