Resource data sheet
DNA Bank Top

pGEMHE-mHec1-sfGFP (#RDB20285)

Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yoshida, S., Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. Fused with sfGFP.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37℃
Gene/insert name mouse Ndc80 cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。

Catalog # Resource name Shipping form Fee
RDB20285 pGEMHE-mHec1-sfGFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pGEMHE-mHec1-sfGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20285).

Reference section:

Yoshida, S., Kaido, M., Kitajima, T.S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [PubMed] [Article] [RRC of NBRP]