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pGEMHE-mHec1-sfGFP (#RDB20285)

Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.


Drawn by SnapGene® software
Sequence (full) RDB20285hts01.seq
Publication Yoshida, S., Dev. Cell 33 (5): 589-602 (2015). [link to RRC of NBRP]
Test sheet RDB20285_B4Enp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. Fused with sfGFP.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Ndc80 cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。

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RDB20285 pGEMHE-mHec1-sfGFP DNA solution


How to cite this biological resource

Materials & Methods section:

The pGEMHE-mHec1-sfGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20285).

Reference section:

Yoshida, S., Kaido, M., Kitajima, T.S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]