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pENTR4-GFP-DcpS-BGHpA (#RDB20276)

A Gateway entry vector of mouse Dcps N-terminally tagged with EGFP.

Clone info. A Gateway entry vector of mouse Dcps N-terminally tagged with EGFP. It also contains bovine growth hormone polyadenylation sequence downstream of the cDNA.
Comment protein expression was confirmed by Western blotting after transferring to an destination vector (pINDUCER20).
Vector backbone pENTR4 (plasmid)
Growth conditions LB+Kanamycin, 37oC
Growth remarks ccdB Survival
Gene/insert name mouse Dcps cDNA; Aequorea victoria GFP cDNA.
Depositor|Developer Katahira, Jun |
 
Sequence (full) RDB20276hts01.seq checkAssembled from experimentally sequenced data.
Publication Katahira, J., Nucleic Acids Res. 51 (14): 7602-7618 (2023). [link to RRC of NBRP]
Test sheet RDB20276C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Katahira, J. et al., Nucleic Acids Research, 51, 7602-7618, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Osaka Metropolitan University.
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること（Katahira, J. et al., Nucleic Acids Research, 51, 7602-7618, 2023）。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、大阪公立大学の許可を得ること。
備考
Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20276 pENTR4-GFP-DcpS-BGHpA DNA solution

How to cite this biological resource

Materials & Methods section:

The pENTR4-GFP-DcpS-BGHpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20276).

Reference section:

Katahira, J., Ohmae, T., Yasugi, M., Sasaki, R., Itoh, Y., Kohda, T., Hieda, M., Yokota Hirai, M., Okamoto, T., Miyamoto, Y., Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex. Nucleic Acids Res. 51 (14): 7602-7618 (2023). PMID 37260089. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Katahira, J., Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex. Nucleic Acids Res. 51 (14): 7602-7618 (2023). PMID 37260089. [link to RRC of NBRP]