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mTmc2 pEGFPC1 (#RDB20273)

Expression vector of mouse transmembrane channel like 2 (Tmc2), EGFP-tag at N-terminus.

Clone info. Expression vector of mouse transmembrane channel like 2 (Tmc2), EGFP-tag at N-terminus.
Vector backbone pEGFPC1 (plasmid)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Tmc2 cDNA; Aequorea victoria GFP cDNA.
Depositor|Developer Yamaguchi, Soichiro |
 
Sequence (full) RDB20273hts01.seq checkAssembled from experimentally sequenced data.
Publication Yamaguchi, S., PLoS One 18 (6): e0287249 (2023) [link to RRC of NBRP]
Test sheet RDB20273C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Yamaguchi S., et al. PLoS One, 18(6): e0287249, 2023.)
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用を必要とする (Yamaguchi S., et al. Int. J. Mol. Sci. ;21(18): 6465, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20273 mTmc2 pEGFPC1 DNA solution

How to cite this biological resource

Materials & Methods section:

The mTmc2 pEGFPC1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20273).

Reference section:

Yamaguchi, S., Kamino, M., Hamamura, M., Otsuguro, K.I., The cytosolic N-terminal region of heterologously-expressed transmembrane channel-like protein 1 (TMC1) can be cleaved in HEK293 cells. PLoS One 18 (6): e0287249 (2023) PMID 37352201. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yamaguchi, S., The cytosolic N-terminal region of heterologously-expressed transmembrane channel-like protein 1 (TMC1) can be cleaved in HEK293 cells. PLoS One 18 (6): e0287249 (2023) PMID 37352201. [link to RRC of NBRP]