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pEF-BOS-anti human CD271-#16 (#RDB20193)

Expression vector of anti-human CD271.

Clone info. Expression vector of anti-human CD271. See, Morita, S. et al., Cancer Lett., 461: 144-152, 2019. Please note that the anti-human CD271 coding region was transfer to pEF-BOS from pcDNA3.4.
Comment Expression was confirmed by the depositor with flowcytometory.
Vector backbone pEF-BOS (plasmid)
Gene/insert name moue IgG; human IgG; Woodchuck hepatitis virus posttranscriptional regulatory element (WRPE); HSV TK poly(A) signal; CMV promoter.
Depositor|Developer Mochizuki, Mai | Morita, Shinkichi |
 
Sequence (full) RDB20193hts01.seq checkAssembled from experimentally sequenced data.
Publication Morita, S., Cancer Lett. 461: 144-152 (2019). [link to RRC of NBRP]
Test sheet RDB20193_B3Kgp1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morita S. et al., Cancer Letters, 461, 144-152, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Miyagi Cancer Center Research Institute.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Morita S. et al., Cancer Letters, 461, 144-152, 2019)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、宮城県立がんセンター研究所の許可を得ること。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20193 pEF-BOS-anti human CD271-#16 DNA solution

How to cite this biological resource

Materials & Methods section:

The pEF-BOS-anti human CD271-#16 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20193).

Reference section:

Morita, S., Mochizuki, M., Wada, K., Shibuya, R., Nakamura, M., Yamaguchi, K., Yamazaki, T., Imai, T., Asada, Y., Matsuura, K., Sugamura, K., Katori, Y., Satoh, K., Tamai, K., Humanized anti-CD271 monoclonal antibody exerts an anti-tumor effect by depleting cancer stem cells. Cancer Lett. 461: 144-152 (2019). PMID 31325530. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Morita, S., Humanized anti-CD271 monoclonal antibody exerts an anti-tumor effect by depleting cancer stem cells. Cancer Lett. 461: 144-152 (2019). PMID 31325530. [link to RRC of NBRP]