RDB20187 - pCE-hSK(L507A)

pCE-hSK(L507A) (#RDB20187)

Expression vector of human SOX2 and KLF4 (L507A mutant) for generating iPS cells.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Clone info.	Expression vector of human SOX2 and KLF4 (L507A mutant) for generating iPS cells.
Vector backbone	pCE (plasmid)
Growth conditions	LB+Amp, 37℃
Gene/insert name	human SOX2 cDNA, KLF4 cDNA.
Depositor\|Developer	Hayashi, Yohei \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR is requested (Describe by RIKEN BRC after publication).
Additional terms and conditions:
The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE is a modified material of pCE-hSK (Plasmid #41814) developed by Dr. Shinya Yamanaka of the CiRA, Kyoto University, and deposited at Addgene. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by Dr. Yamanaka (Okita, K. et al., Stem Cells 31(3):458-466, 2013) is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR is requested (Describe by RIKEN BRC after publication). Additional terms and conditions: The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. The RECIPIENT acknowledges that the BIOLOGICAL RESOURCE is a modified material of pCE-hSK (Plasmid #41814) developed by Dr. Shinya Yamanaka of the CiRA, Kyoto University, and deposited at Addgene. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by Dr. Yamanaka (Okita, K. et al., Stem Cells 31(3):458-466, 2013) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (決定次第理研BRCで記載)。付加的使用条件：本件リソースは非営利機関の学術研究目的にのみ利用することができる。本件リソースは、京都大学 iPS 細胞研究所(CiRA)の山中伸弥博士が開発し、Addgene に寄託した pCE-hSK (Plasmid #41814)をもとに改変されたプラスミドである。利用者は、研究成果の公表にあたって山中伸弥博士が指定する文献 (Okita, K. et al., Stem Cells 31(3):458-466, 2013)を引用すること。 CAGプロモータを含むリソースについて: Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991 を引用すること。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (決定次第理研BRCで記載)。
付加的使用条件：
本件リソースは非営利機関の学術研究目的にのみ利用することができる。
本件リソースは、京都大学 iPS 細胞研究所(CiRA)の山中伸弥博士が開発し、Addgene に寄託した pCE-hSK (Plasmid #41814)をもとに改変されたプラスミドである。利用者は、研究成果の公表にあたって山中伸弥博士が指定する文献 (Okita, K. et al., Stem Cells 31(3):458-466, 2013)を引用すること。
CAGプロモータを含むリソースについて: Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991 を引用すること。

Catalog #	Resource name	Shipping form	Fee
RDB20187	pCE-hSK(L507A)	DNA solution

How to cite this biological resource

Materials & Methods section:

The pCE-hSK(L507A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20187).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

External Database
human SOX2

human KLF4

Reference sequence