Retroviral vector for expression of human ATP11B ATPase-deficient mutant (E180Q) in mammalian cells, C-terminal HA-tag.
Drawn by SnapGene® software |
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Clone info. | Retroviral vector for expression of human ATP11B ATPase-deficient mutant (E180Q) in mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088) |
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Vector backbone | pMX (plasmid) |
Gene/insert name | human ATP11B cDNA; HA-tag. |
Depositor|Developer | Shin, Hye-Won | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tone, T. et al., FEBS Lett. 594(3): 412-423, 2020) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tone, T. et al., FEBS Lett. 594(3): 412-423, 2020) 。非営利学術機関の学術研究に限る。 MTAに書く付加的使用条件: pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB20184 | pMx/ATP11B(E180Q)-HA | DNA solution |
Materials & Methods section:
The pMx/ATP11B(E180Q)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20184). |
Reference section:
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