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pMx/ATP10B(E210Q)-HA (#RDB20181)

Retroviral vector for expression of human ATP10B ATPase-deficient mutant (E210Q) in mammalian cells, C-terminal HA-tag.


Drawn by SnapGene® software
Sequence (full) RDB20181hts01.seq
Publication Naito, T., J. Biol. Chem. 290 (24): 15004-15017 (2015). [link to RRC of NBRP]
Test sheet RDB20181_B3Kfp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Retroviral vector for expression of human ATP10B ATPase-deficient mutant (E210Q) in mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088)
Vector backbone pMX (plasmid)
Gene/insert name human ATP10B cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Naito, T. et al., J. Biol. Chem. 290(24): 15004-17, 2015) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Naito, T. et al., J. Biol. Chem. 290(24): 15004-17, 2015)。非営利学術機関の学術研究に限る。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20181 pMx/ATP10B(E210Q)-HA DNA solution

How to cite this biological resource

Materials & Methods section:

The pMx/ATP10B(E210Q)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20181).

Reference section:

Naito, T., Takatsu, H., Miyano, R., Takada, N., Nakayama, K., Shin, H.W., Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is Involved in Plasma Membrane Dynamics. J. Biol. Chem. 290 (24): 15004-15017 (2015). PMID 25947375. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Naito, T., Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is Involved in Plasma Membrane Dynamics. J. Biol. Chem. 290 (24): 15004-15017 (2015). PMID 25947375. [link to RRC of NBRP]