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pCAG/ATP8B4(E170Q)-HA (#RDB20165)

Expression vector of human ATP8B4 ATPase-deficient mutant (E170Q) for mammalian cells, C-terminal HA-tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Takatsu, H., J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human ATP8B4 ATPase-deficient mutant (E170Q) for mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with pcDNA3/HF-CDC50A (RDB20145) to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088)
Vector backbone pCAG (plasmid)
Growth conditions LB+Amp, 37℃
Gene/insert name human ATP8B4 cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014)。非営利学術機関の学術研究に限る。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB20165 pCAG/ATP8B4(E170Q)-HA DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG/ATP8B4(E170Q)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20165).

Reference section:

Takatsu, H., Tanaka, G., Segawa, K., Suzuki, J., Nagata, S., Nakayama, K., Shin, H.W., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takatsu, H., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [PubMed] [Article] [RRC of NBRP]