Expression vector of human ATP8B2 ATPase-deficient mutant (E171Q) for mammalian cells, C-terminal HA-tag.
Drawn by SnapGene® software |
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Clone info. | Expression vector of human ATP8B2 ATPase-deficient mutant (E171Q) for mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088) |
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Vector backbone | pCAG (plasmid) |
Gene/insert name | human ATP8B2 cDNA; HA-tag. |
Depositor|Developer | Shin, Hye-Won | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takatsu, H. et al., J. Biol. Chem. 289(48): 33543-33556, 2014)。非営利学術機関の学術研究に限る。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB20164 | pCAG/ATP8B2(E171Q)-HA | DNA solution |
Materials & Methods section:
The pCAG/ATP8B2(E171Q)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20164). |
Reference section:
Takatsu, H., Tanaka, G., Segawa, K., Suzuki, J., Nagata, S., Nakayama, K., Shin, H.W., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Takatsu, H., Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. J. Biol. Chem. 289 (48): 33543-33556 (2014). PMID 25315773. [link to RRC of NBRP] |
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