Resource data sheet
DNA Bank Top

pCAG/ATP11C-b-HA (#RDB20143)

Expression vector of human ATP11Cb for mammalian cells, C-terminal HA-tag.


Drawn by SnapGene® software
Sequence (full) RDB20143hts01.seq
Publication Inoue, H., J. Cell Sci. 134 (20): jcs258523 (2021). [link to RRC of NBRP]
Test sheet RDB20143_B3Kfp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of human ATP11Cb isoform for mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088)
Vector backbone pCAG (plasmid)
Gene/insert name human ATP11C (splice variant ATP11C-b) cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inoue, H. et al., J. Cell Sci. 134(20): jcs258523, 2021) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inoue, H. et al., J. Cell Sci. 134(20): jcs258523, 2021)。非営利学術機関の学術研究に限る。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20143 pCAG/ATP11C-b-HA DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG/ATP11C-b-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20143).

Reference section:

Inoue, H., Takatsu, H., Hamamoto, A., Takayama, M., Nakabuchi, R., Muranaka, Y., Yagi, T., Nakayama, K., Shin, H.W., The interaction of ATP11C-b with ezrin contributes to its polarized localization. J. Cell Sci. 134 (20): jcs258523 (2021). PMID 34528675. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inoue, H., The interaction of ATP11C-b with ezrin contributes to its polarized localization. J. Cell Sci. 134 (20): jcs258523 (2021). PMID 34528675. [link to RRC of NBRP]